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Published on: February 1, 2018
Amperometric lysine bioprobes analysis in feeds
M G Lavagnini1, D Moscone, G Palleschi
1Dipartimento di Scienze e Tecnologie Chimiche Universita' di Roma Tor Vergata Via della Ricerca Scientifica 00133 Rome, Italy.
Talanta
|August 1, 1993
Summary
New amperometric enzyme electrode probes enable specific L-lysine determination. These stable, reproducible bioprobes offer rapid analysis in batch, flow, and flow injection analysis (FIA) for feed applications.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Amperometric enzyme electrodes are crucial for detecting specific biomolecules.
- L-lysine determination is important in various fields, including animal feed analysis.
- Existing methods may lack specificity, speed, or stability.
Purpose of the Study:
- To develop and characterize a novel amperometric enzyme electrode for L-lysine determination.
- To optimize the electrode's performance for stability, reproducibility, and sensitivity.
- To evaluate the bioprobe's efficacy in both batch and flow analysis, including feed samples.
Main Methods:
- Immobilization of lysine oxidase enzyme onto a platinum electrode using a preactivated polymer support.
- Incorporation of blocking membranes to enhance stability and prevent interferences.
- Optimization of operational parameters such as pH, temperature, and storage.
- Validation using flow injection analysis (FIA) and comparison with High-Performance Liquid Chromatography (HPLC).
Main Results:
- The developed L-lysine bioprobe demonstrated high sensitivity with a detection limit of 5 x 10(-7)M.
- Rapid response times were achieved: ~2 min for batch/flow and 30 sec for FIA.
- Probes exhibited excellent stability, remaining functional for over three months and >300 analyses.
- Analysis of lysine in feed samples showed good correlation between the bioprobe and HPLC methods.
Conclusions:
- The novel amperometric enzyme electrode provides a stable, reproducible, and sensitive platform for L-lysine quantification.
- The bioprobe is suitable for diverse analytical applications, including rapid analysis in feed matrices.
- This technology offers a viable alternative to conventional methods for L-lysine determination.
