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Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

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Published on: January 1, 2016

Measuring estrogens using flow injection immunoanalysis with liposome amplification.

L Locascio-Brown¹, S J Choquette

¹National Institute of Standards and Technology, Gaithersburg MD 20899 U.S.A.

|December 1, 1993

Summary

This summary is machine-generated.

This study introduces a new flow injection analysis method for measuring 17-beta-estradiol. The assay uses immobilized antigen and antibody-conjugated liposomes, showing concentration-dependent results for hormone detection.

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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Area of Science:

Analytical Chemistry
Biochemistry
Immunology

Background:

Accurate measurement of hormones like 17-beta-estradiol is crucial in diagnostics.
Existing immunoassay methods can be time-consuming or require complex sample preparation.

Purpose of the Study:

To develop a rapid and sensitive solid-phase competitive immunoassay for 17-beta-estradiol measurement.
To adapt flow injection analysis (FIA) for on-column immunoassays.

Main Methods:

A flow injection analysis system was designed with a column reactor packed with silica particles.
The antigen 17-beta-estradiol was covalently immobilized onto the silica support.
Antibodies were conjugated to liposomes via a streptavidin-biotin linkage.
Competitive binding between sample 17-beta-estradiol and immobilized antigen for antibody sites on liposomes was measured.

Main Results:

The assay demonstrated a concentration-dependent reduction in liposome binding to the solid support.
Sequential immunoassays were successfully performed on-column.
The method offers a novel approach for hormone quantification.

Conclusions:

The developed FIA-based solid-phase immunoassay is a viable method for quantifying 17-beta-estradiol.
This technique simplifies hormone measurement with potential for automation and high throughput.