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Updated: Jun 28, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Flow-injection analysis-spectrophotometric determination of nitrite and nitrate in water samples by reaction with
R Segarra Guerrero1, C Goméz Benito, J Martínez Calatayud
1Departamento de Química Analitica, Universitat de Valéncia, Valéncia, Spain.
Abstract:
A flow-injection manifold is proposed for determination of nitrite based on the reaction with 3,6-diamino acridine (proflavin sulfate) in hydrochloride acid medium. The assembly is adapted for nitrate determination by including a reductive column filled with copperized cadmium. The influence of foreign substances is also studied. The method gives a linear calibration graph over the range 0.06-4 mg 1(-1) nitrite, with an RSD <0.5%. The method was applied to nitrite and nitrate determinations in either waste water or coastal marine water samples.
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