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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
Published on: February 14, 2017
Continuous flow system for the evaluation of the extrinsic coagulation pathway
Talanta
|September 1, 1996
Summary
A new flow injection method reliably measures the extrinsic coagulation pathway by monitoring clot formation. This technique accurately assesses fibrin clotting capacity in human plasma for clinical diagnostics.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Analytical Chemistry
Background:
- The extrinsic coagulation pathway is crucial for hemostasis.
- Accurate evaluation of coagulation factors is essential for diagnosing bleeding disorders.
- Existing methods may have limitations in speed or precision.
Purpose of the Study:
- To develop a simple and reliable method for evaluating the extrinsic coagulation pathway.
- To utilize a flow injection system for real-time monitoring of coagulation.
- To assess the clinical utility of the developed method for fibrin clotting capacity determination.
Main Methods:
- Developed a flow injection system using merging-zones and stopped-flow modes.
- Monitored the final step of coagulation photometrically at 340 nm.
- Measured clotting reaction rates based on the activity of coagulation factors.
Main Results:
- Established a linear range of 10-100% for total clottable activity.
- Achieved a relative standard deviation between 0.9% and 2.8%.
- Demonstrated excellent agreement with a conventional automated nephelometric method.
Conclusions:
- The developed flow injection method provides a simple, reliable, and accurate means for evaluating the extrinsic coagulation pathway.
- The method is suitable for determining fibrin clotting capacity in human plasma from both healthy and diseased individuals.
- This technique offers a valuable tool for clinical diagnostics and coagulation research.
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