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Published on: July 28, 2021
A consideration of electrode polishing prior to application of pulsed amperometric detection
A Inoue1, R L Earley, M W Lehmann
1Department of Chemistry, Knox College, Galesburg, IL 61401 USA.
Abstract:
An examination was made of the effect of electrode polishing on the direct pulsed amperometric detection (PAD) and indirect PAD response to penicillin G. The polishing procedure produced a temporary loss of sensitivity for both types of PAD, which over time relaxed back to higher levels. A temporary increase in the electrode kinetics can be observed as well, particularly for a PAD waveform in the crossover region between direct and indirect PAD. In extreme cases, the penicillin G response can undergo a complete polarity reversal. By observing post-polish equilibration times, it was recommended that the direct and indirect PAD user observe 3 and 5 h delay times, respectively, to insure stable electrode response following an electrode polish.
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