Tetraphenylborate doped polyaniline based novel pH sensor and solid-state urea biosensor

Area of Science:

• Electrochemistry
• Materials Science
• Analytical Chemistry

Background:

• Developing versatile pH sensors for diverse media is crucial.
• Polymer-modified electrodes offer unique sensing capabilities.

Purpose of the Study:

• To report a novel polymer-modified electrode for pH sensing in aqueous and non-aqueous solutions.
• To develop a urea biosensor utilizing this polymer-modified electrode.

Main Methods:

• Electrochemical polymerization of aniline to create a polymer-modified electrode.
• Characterization using Scanning Electron Microscopy (SEM).
• Testing pH sensing with acetic acid and ammonium hydroxide in various media; constructing a urea biosensor with immobilized urease.

Main Results:

• The polymer-modified electrode demonstrated pH sensing capabilities in both aqueous and non-aqueous environments.
• A pH sensitivity slope of approximately 86 mV/pH was achieved.
• The developed urea biosensor exhibited a maximum response of 160 mV at 25°C with a detection limit of 20 μM.

Conclusions:

• The novel polymer-modified electrode is effective for pH sensing across different mediums.
• The electrode serves as a robust platform for developing sensitive urea biosensors.