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Updated: Jun 28, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
Multi-syringe flow injection system with in-line microwave digestion for the determination of phosphorus
M Inês G S Almeida1, Marcela A Segundo, José L F C Lima
1Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Abstract:
A multi-syringe system for spectrophotometric determination of total phosphorus involving in-line digestion is proposed. Sample and digestion solution were dispensed and directed towards a digestion vessel located inside a domestic microwave oven (MWO) where sample digestion took place. Afterwards, the digested sample was merged with the necessary reagents for the colorimetric determination based on the molybdenum blue method. Several digestion conditions were studied regarding composition of digestion solution, digestion time and power set on the MWO. The system was applied to waste water samples and results shown a good agreement with the reference method. Repeatable results (R.S.D.<2.41%) and determination frequency of 12h(-1) were obtained.
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