Related Experiment Video
Updated: Jun 28, 2026
Growing Neural Stem Cells from Conventional and Nonconventional Regions of the Adult Rodent Brain
Published on: November 18, 2013
[The study on growth and proliferation of neural stem cells from rats in vitro]
Qingguo Chen1, Yong Fu, Xianhong Wang
1Department of Otolaryngology, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.
Objective:
To investigate culturing neural stem cells (NSCs) from rat embryos in vitro and to observe their growth and differentiation.
Method:
NSCs were isolated from hippocampus of SD rat embryos (P16-P18) and cultured in DMEM/F12 medium containing EGF, bFGF, B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation.
Result:
NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive.
Conclusion:
NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.
