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Updated: Jun 28, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Electrochemical and spectroscopic evidences of the interaction between DNA and Pt(II)(dppf)-complex
Lucilene D Mello1, Emerson S Ribeiro, Lauro T Kubota
1Centro de Ciências Exatas e Tecnológicas, Universidade Federal do Pampa, Campus Bagé, Bage, RS, Brazil.
Abstract:
The interaction of Pt(II)(dppf)-complex, namely [Pt(dppf)(H(2)O)(2)](2+) with DNA was investigated by DPV and (1)H-NMR techniques. The results showed that the interaction process has been characterized by changes in the electrochemical parameters of both compounds and the formation of a new anodic current peak close to the anodic current peak of the [Pt(dppf)(H(2)O)(2)](2+). In addition, the (1)H-NMR spectra show that the coordination of Pt(II)(dppf)-complex to dsDNA occurs via N(7) of guanine. Others parameters like pH and ionic strength that affect the interaction process were also investigated.
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