Related Experiment Video
Updated: Jun 28, 2026

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
Published on: August 11, 2021
Inhibitory effects of noscapine on dopamine biosynthesis in PC12 cells
1College of Pharmacy, Chungbuk National University, San 48, Kaeshin-Dong, 361-763, Cheongju, Korea.
Abstract:
The effects of noscapine, a phthalide isoquinoline alkaloid, on dopamine biosynthesis and tyrosine hydroxylase (TH) activity in PC12 cells were investigated. Noscapine showed 74.6% inhibition on dopamine content in PC12 cells at a concentration of 20 muM. IC(50) of noscapine was 6.8 muM. TH activity was inhibited by the treatment of noscapine in PC12 cells (20.9% inhibition at 20 muM). Therefore, the inhibition of TH activity by noscapine might be involved in at least one component of the reduction of dopamine biosynthesis in PC12 cells.
Related Concept Videos
Drugs Affecting Neurotransmitter Synthesis
Drugs Affecting Neurotransmitter Release or Uptake
Neurochemical Transmission: Sites of Drug Action