Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Separation and Characterization of Plastic Waste Packaging Contaminated with Food Residues.

Polymers·2023
Same author

Response to Xiao et al.: If everything is a loot box, nothing is.

Addiction (Abingdon, England)·2022
Same author

Links between problem gambling and spending on booster packs in collectible card games: A conceptual replication of research on loot boxes.

PloS one·2021
Same author

Nothing else matters: Video games create sustained attentional selection away from task-irrelevant features.

Attention, perception & psychophysics·2020
Same author

Image-based search and retrieval for biface artefacts using features capturing archaeologically significant characteristics.

Machine vision and applications·2020
Same author

The prevalence of loot boxes in mobile and desktop games.

Addiction (Abingdon, England)·2020
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

5'-azacytidine expression arrays.

Paul Cairns1

  • 1Department of Surgical Oncology, Fox Chase Cancer Center, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2008
PubMed
Summary
This summary is machine-generated.

DNA methylation inhibitor azacytidine can reverse gene silencing in cancer cells. This approach helps identify new hypermethylated genes and pathways across various cancer types.

More Related Videos

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Related Experiment Videos

Last Updated: May 8, 2026

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Pharmacology

Background:

  • Epigenetic silencing of genes plays a crucial role in cancer development.
  • DNA methylation is a key epigenetic mechanism, and its inhibitors can reverse gene silencing.
  • Traditional methods for analyzing gene methylation in cancer have limitations in genome-wide scope and identifying transcriptionally relevant changes.

Purpose of the Study:

  • To investigate the use of DNA methylation inhibitors for identifying epigenetically silenced genes in cancer.
  • To leverage expression microarray-based screening coupled with a reactivation strategy for genome-wide analysis.
  • To establish a validation strategy for accurate identification of methylated genes in primary tumors.

Main Methods:

  • Utilizing 5-Aza-2'-deoxycytidine (azacytidine) to inhibit DNA methyltransferase activity and induce gene reactivation.
  • Employing expression microarray-based screening for genome-wide analysis of gene expression changes.
  • Implementing a validation strategy involving analysis of primary tumors to confirm findings from cell lines.

Main Results:

  • Azacytidine treatment leads to generalized demethylation and reactivation of epigenetically silenced genes.
  • Expression microarray screening coupled with reactivation preferentially identifies reexpressed genes.
  • Validation in primary tumors is essential due to potential downstream deregulation caused by azacytidine.

Conclusions:

  • Azacytidine-based reactivation is a powerful strategy for identifying epigenetically silenced genes in cancer.
  • This approach facilitates the discovery of novel hypermethylated genes and pathways in diverse cancer types.
  • Combining cell line studies with primary tumor validation enhances the reliability of findings.