Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 28, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
07:48

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

"System 48" high-throughput cloning and protein expression analysis.

James M Abdullah1, Andrzej Joachimiak, Frank R Collart

  • 1Biosciences Division, Argonne National Laboratory, Lemont, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 7, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Phylogenetic Analysis and Structural Evaluation of <i>Staphylococcus aureus</i> Serine-Aspartate Repeat-Containing Protein D with a Focus on Periprosthetic Joint Infection.

bioRxiv : the preprint server for biology·2026
Same author

Protein structure of a glycoside hydrolase family 30, subfamily 12 endo-1,4-β-xylanase.

Acta crystallographica. Section D, Structural biology·2026
Same author

Elongation Factor Tu Acts as a Chaperone to Activate an Antibacterial RNase Toxin.

Molecular microbiology·2026
Same author

PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders.

bioRxiv : the preprint server for biology·2025
Same author

Functional and Structural Studies on the Esperamicin Thioesterase and Progress toward Understanding Enediyne Core Biosynthesis.

Journal of natural products·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

This study introduces an efficient, high-throughput cloning and expression strategy using 48- and 96-well plates for generating validated expression clones in Escherichia coli, reducing time and material needs.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Structural Genomics

Background:

  • High-throughput generation of validated expression clones is crucial for structural genomics and functional studies.
  • Traditional cloning and colony selection methods can be labor-intensive and time-consuming, limiting throughput.

Purpose of the Study:

  • To develop and validate an efficient, plate-based cloning and expression strategy for high-throughput clone generation.
  • To integrate 48- and 96-well plate formats throughout the cloning and colony selection process.
  • To reduce the time and material requirements for producing validated expression clones.

Main Methods:

  • Implementation of a 48- or 96-well plate format for all cloning and colony selection stages.
  • Integration of a 48-grid agar growth plate for enhanced colony selection throughput.

More Related Videos

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Related Experiment Videos

Last Updated: Jun 28, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
07:48

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

  • Compatibility with automated liquid handlers and multichannel pipettes for streamlined workflow.

    • Main Results:

    • Significant increase in throughput for generating validated expression clones.
    • Substantial reduction in both time and material costs associated with the cloning pipeline.
    • Successful validation through the production and screening of several thousand clones.

    Conclusions:

    • The described plate-based strategy offers an efficient and scalable method for high-throughput clone generation.
    • This approach streamlines the cloning and expression pipeline, making it suitable for large-scale structural genomics projects.
    • The optimized workflow enhances productivity while minimizing resource utilization.