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UGA can be decoded as tryptophan at low efficiency in Bacillus subtilis
P S Lovett1, N P Ambulos, W Mulbry
1Department of Biological Sciences, University of Maryland-Baltimore County, Catonsville 21228-5398.
Journal of Bacteriology
|March 11, 1991
Abstract:
Replacement of cat-86 codon 7 or 144 with the UGA codon permitted the gene to confer chloramphenicol resistance in wild-type Bacillus subtilis. UAA replacements of the same codons resulted in a chloramphenicol-sensitive phenotype in wild-type B. subtilis and a chloramphenicol-resistant phenotype in suppressor-positive strains. N-terminal sequencing showed that UGA at codon 7 was decoded as tryptophan in wild-type cells, at an efficiency of about 6%.