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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Stable isotopic labeling in proteomics.

Kris Gevaert1, Francis Impens, Bart Ghesquière

  • 1Department of Medical Protein Research, VIB, Ghent University, Ghent, Belgium.

Proteomics
|November 13, 2008
PubMed
Summary

Stable isotope labeling using deuterium, carbon-13, nitrogen-15, and oxygen-18 is crucial for quantitative proteomics. This review covers metabolic and chemical labeling methods for analyzing protein expression and modifications.

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Last Updated: Jun 28, 2026

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)

Published on: May 1, 2017

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water
09:43

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Published on: February 20, 2013

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Stable isotope labeling is a cornerstone technique in quantitative proteomics.
  • Isotopes like deuterium, carbon-13, nitrogen-15, and oxygen-18 are incorporated into proteins and peptides.
  • These labels enable precise quantification using mass spectrometry.

Purpose of the Study:

  • To review common isotope labeling strategies in proteomics.
  • To discuss applications in differential protein profiling and modification analysis.
  • To highlight the role of mass spectrometry in isotope-based quantification.

Main Methods:

  • Metabolic labeling in cells and organisms.
  • Postmetabolic chemical or enzymatic labeling of proteins and peptides.
  • Mass spectrometry for segregation and quantification of light and heavy labeled ions.

Main Results:

  • Stable isotope labeling allows for accurate quantitative differential protein profiling.
  • Targeted labeling strategies facilitate the monitoring of dynamic proteome changes.
  • Analysis of protein modifications is enhanced through specific labeling techniques.

Conclusions:

  • Isotope labeling is indispensable for advanced quantitative proteomics.
  • Diverse labeling strategies cater to various proteomic research questions.
  • Mass spectrometry-based quantification of labeled peptides provides deep proteome insights.