Related Experiment Video
Updated: Jun 28, 2026

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures
Published on: February 10, 2011
[Genetic identification as method of detection of pathogenic and symbiotic strains of enterococci]
Abstract:
Comparative study of 97 cultures of enterococci, including reference highly virulent strain Enterococcus faecalis ATCC 259212, 35 clinical isolates of E. faecium, 58 isolates of E. faecalis, and 3 probiotic strains of E. faecium: Linex (Lek company, Slovenia), SF68 (Bifiform, Denmark), and L3 (Avena, Russia). Thirteen of 93 cultures were isolated from patients treated with probiotic Linex, containing E. faecium (Linex) bacterium. Comparative analysis of cultures on the presence of 8 genes determining virulence (esp, asa1, efaA, cylA, cylM, gelE, sprE, and fsrB) was performed using polymerase chain reaction and testing with antibiotics. It was established that in some cases clinical cultures of enterococci used for analysis carried genes of pathogenicity and were not related to E. faecium (Linex) strain included in composition of the probiotic drug.
Related Concept Videos
Methods of Classification and Identification
Modern Molecular Taxonomy
Applications of Molecular Taxonomy
Rapid Identification of Pathogens
Differential Staining Technique
Automated Microbial Diagnostics

