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Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
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Published on: February 11, 2009

Plasma soluble e-selectin in necrotising enterocolitis.

A K Khoo1, N J Hall, N Alexander

  • 1Department of Paediatric Surgery, UCL Institute of Child Health and Great Ormond Street Hospital for Children NHS Trust, London, UK.

European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [Et Al] = Zeitschrift Fur Kinderchirurgie
|November 18, 2008
PubMed
Summary
This summary is machine-generated.

Soluble E-selectin (sE-selectin) levels increase after surgery for necrotising enterocolitis (NEC) in infants. However, prematurity, not NEC severity, appears to drive elevated sE-selectin, limiting its use as a prognostic marker.

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Area of Science:

  • Neonatal immunology
  • Gastroenterology
  • Vascular biology

Background:

  • E-selectin mediates leukocyte-endothelial adhesion and is elevated in necrotising enterocolitis (NEC).
  • Soluble E-selectin (sE-selectin) is a circulating marker of endothelial activation.
  • Increased intestinal E-selectin in NEC correlates with poor outcomes.

Purpose of the Study:

  • To investigate the association between circulating sE-selectin levels and prognosis in infants with NEC.
  • To determine if sE-selectin can serve as a predictive biomarker for NEC severity and patient outcomes.

Main Methods:

  • Plasma samples from 20 infants with Bell stage II/III NEC were analyzed using ELISA.
  • Sequential Organ Failure Assessment (SOFA) scores assessed illness severity.
  • Statistical analysis included Spearman's rank correlation and Wilcoxon signed rank test.

Main Results:

  • Plasma sE-selectin negatively correlated with corrected gestational age (r = -0.425, p = 0.006).
  • No significant association was found between sE-selectin and NEC outcome (survival/death), disease extent, or SOFA score.
  • Surgery for suspected perforation significantly elevated sE-selectin levels (p = 0.031).

Conclusions:

  • Circulating sE-selectin levels rise post-surgery in NEC infants.
  • Prematurity, rather than NEC severity, is linked to increased sE-selectin.
  • sE-selectin's utility as a prognostic biomarker for NEC severity is confounded by prematurity.