Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Multitarget magnetic activated cell sorter.

Jonathan D Adams1, Unyoung Kim, H Tom Soh

  • 1Departments of Physics, Mechanical Engineering, and Materials, University of California, Santa Barbara, CA 93106-5050, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 19, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Generalizable Fluorescence Sensor Platform for Sample Preparation-Free Protein Detection.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Theoretical Framework and Experimental Validation of Multiplexed Analyte Quantification Using Cross-Reactive Affinity Reagents.

Analytical chemistry·2025
Same author

High-Speed Quantitative Nanomechanical Mapping by Photothermal Off-Resonance Atomic Force Microscopy.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

A biochemical sensor with continuous extended stability in vivo.

Nature biomedical engineering·2025
Same author

Direct Optical Detection of Factor Xa Activity in Minimally Processed Whole Blood.

ACS sensors·2025
Same author

Biosensors for Parkinson's Disease: Where Are We Now, and Where Do We Need to Go?

ACS sensors·2024
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

This study introduces a novel multitarget magnetic activated cell sorter (MT-MACS) for simultaneous, high-purity cell sorting. The microfluidics-based system efficiently separates multiple cell types using distinct magnetic tags, overcoming limitations of single-parameter sorting methods.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Sorting

Background:

  • Magnetic cell sorting is crucial in biotechnology but limited to single-parameter separation.
  • Simultaneous sorting of multiple cell types with high purity and throughput remains a challenge.

Purpose of the Study:

  • To develop a microfluidics-based system for simultaneous, multi-target magnetic cell sorting.
  • To enable high-purity, high-throughput separation of multiple cell types in a continuous flow.

Main Methods:

  • Utilized a multitarget magnetic activated cell sorter (MT-MACS) device.
  • Employed microfluidics for controlled laminar flow and patterned ferromagnetic structures for magnetophoresis.
  • Labeled target cells with distinct magnetic tags based on saturation magnetization and size.

More Related Videos

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
04:53

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Published on: April 5, 2022

Related Experiment Videos

Last Updated: Jun 27, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination
04:53

Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

Published on: April 5, 2022

Main Results:

  • Achieved simultaneous sorting of multiple magnetic tags with >90% purity and >5,000-fold enrichment.
  • Demonstrated purification of multiple bacterial cell types with >90% purity and >500-fold enrichment.
  • Operated the system at a throughput of 10^9 cells per hour.

Conclusions:

  • The MT-MACS system enables simultaneous, spatially-addressable sorting of multiple target cell types.
  • This technology overcomes limitations of existing magnetic cell separation methods.
  • Offers potential for advanced applications in diagnostics and cell-based therapies.