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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 27, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Simultaneous multiwavelength laminar optical tomography.

Sean A Burgess1, Matthew B Bouchard, Baohong Yuan

  • 1Laboratory for Functional Optical Imaging, Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA. sab2161@columbia.edu

Optics Letters
|November 19, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a new multiwavelength laminar optical tomography system for rapid, depth-resolved tissue imaging. It overcomes multiplexing issues, enabling high-speed, motion-artifact-free spectroscopic analysis of biological absorbers.

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Area of Science:

  • Biomedical Optics
  • Medical Imaging
  • Optical Tomography

Background:

  • Spatially resolved reflectance measurements offer depth-resolved optical property characterization of superficial tissues.
  • Previous methods faced challenges in rapid multiwavelength data acquisition due to multiplexing problems.

Purpose of the Study:

  • To report a novel multiwavelength laminar optical tomography system.
  • To enable rapid, in vivo, depth-resolved spectroscopic imaging unaffected by motion artifacts.

Main Methods:

  • Developed a multiwavelength laminar optical tomography system.
  • The system acquires data from multiple source-detector separations at three wavelengths simultaneously.
  • Phantom validation studies were conducted.

Main Results:

  • The system achieves frame rates exceeding 100 Hz.
  • Enables motion-artifact-free imaging.
  • Facilitates depth-resolved spectroscopic imaging of absorbers like oxy- and deoxyhemoglobin or multiple fluorophores.

Conclusions:

  • The novel system overcomes previous limitations in multiwavelength data acquisition.
  • It provides a powerful tool for high-speed, in vivo optical tomography.
  • The technology has potential for advanced biomedical imaging applications.