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Related Concept Videos

Classification of Leukocytes01:30

Classification of Leukocytes

Leukocytes are classified into two groups based on the presence or absence of cytoplasmic granules. Granular leukocytes, which contain granules, belong to the myeloid lineage and are divided into three subtypes: neutrophils, eosinophils, and basophils. These cells are roughly spherical and characterized by the granules in their cytoplasm.
Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
The Nucleus01:32

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
The Nucleus01:25

The Nucleus

The nucleus is a membrane-bound organelle that acts as a control center in a eukaryotic cell. It contains chromosomal DNA, which controls gene expression and precisely regulates the production of proteins within the cell. In contrast, the DNA inside the mitochondria and chloroplast only carries out functions that are specific to those organelles.
Arrangement of DNA within Nucleus
The regulation of gene expression inside the nucleus is dependent on many factors, including the DNA structure. The...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...

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In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
08:15

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy

Published on: September 8, 2021

The LINC-less granulocyte nucleus.

Ada L Olins1, Thanh V Hoang, Monika Zwerger

  • 1Department of Biology, Bowdoin College, Brunswick, ME 04011, USA. dolins@bowdoin.edu

European Journal of Cell Biology
|November 21, 2008
PubMed
Summary
This summary is machine-generated.

Neutrophils, crucial for fighting infections, are highly adaptable cells. This study reveals a lack of certain nuclear proteins in neutrophils, supporting the hypothesis that this facilitates their ability to deform and navigate infection sites.

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The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

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Related Experiment Videos

Last Updated: Jun 27, 2026

In Situ Exploration of Murine Megakaryopoiesis using Transmission Electron Microscopy
08:15

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Published on: September 8, 2021

Selective Harvesting of Marginating-pulmonary Leukocytes
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The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood
07:17

The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood

Published on: February 7, 2025

Area of Science:

  • Cell Biology
  • Immunology
  • Molecular Biology

Background:

  • Neutrophils are vital immune cells rapidly recruited to infection sites.
  • Their ability to deform and migrate through tissues is critical for function.
  • The nuclear envelope's interaction with the cytoskeleton (LINC complex) influences cell mechanics.

Purpose of the Study:

  • To investigate the presence and composition of the LINC complex in differentiated human immune cells.
  • To compare LINC complex protein expression in granulocytic versus macrophage states.
  • To explore the relationship between LINC complex components and granulocyte cellular malleability.

Main Methods:

  • Analysis of LINC complex proteins (nesprin, SUN, plectin, vimentin) in HL-60 cell line (granulocytic, monocytic, macrophage states).
  • Examination of LINC complex proteins in primary human blood leukocytes, including monocyte-derived macrophages.
  • Comparative analysis of protein expression across different cell differentiation states.

Main Results:

  • HL-60 granulocytes showed a deficiency in nesprin 1 giant, nesprin 2 giant, SUN1, plectin, and vimentin.
  • Macrophage states (HL-60 derived and blood-derived) expressed nesprin 1 giant, plectin, and vimentin.
  • SUN2 was present in the nuclear envelope of both granulocytic and macrophage states.

Conclusions:

  • The paucity of specific LINC complex proteins in granulocytes suggests a mechanism for enhanced cellular deformability.
  • Observed differences in LINC complex composition correlate with cell differentiation and function.
  • These findings support the hypothesis that granulocyte adaptations, including nuclear structure and LINC complex composition, facilitate their migration during infection response.