ATP in current biotechnology: regulation, applications and perspectives

Area of Science:

• Biotechnology
• Metabolic Engineering
• Biochemical Engineering

Background:

• Adenosine tri-phosphate (ATP) is crucial for cellular energy, driving metabolic reactions and supporting industrial strain growth and metabolite production.
• Optimizing ATP levels is key to enhancing efficiency in industrial biotechnology processes.
• Understanding ATP's role is vital for advancing metabolic engineering and bioprocess development.

Purpose of the Study:

• To review current advancements in manipulating ATP in industrial strains.
• To discuss strategies for optimizing ATP levels for improved industrial biotechnology outcomes.
• To elucidate the mechanisms underlying ATP's influence on substrate utilization and stress tolerance.

Main Methods:

• Summarizing and discussing current research on ATP manipulation strategies.
• Analyzing methods to alter NADH availability and regulate NADH oxidation pathways.
• Reviewing the impact of oxygen supply, proton gradient, electron transfer chain, and F(0)F(1)-ATPase activity on ATP levels.

Main Results:

• Application of ATP manipulation strategies has led to optimal product concentrations, yields, and productivity.
• Elucidation of mechanisms by which ATP enhances substrate utilization spectra.
• Demonstration of ATP's role in improving tolerance to harsh environmental conditions.

Conclusions:

• Effective manipulation of ATP is critical for maximizing performance in industrial biotechnology.
• Further research into ATP dynamics can unlock new potential for strain improvement and bioprocess optimization.
• Addressing key issues in ATP manipulation is essential for future advancements in the field.