A novel label-free multi-throughput optical biosensor based on localized surface plasmon resonance.

Area of Science:

• Nanotechnology
• Biosensing
• Optical Physics

Background:

• Label-free detection is crucial for efficient biomolecular analysis.
• Existing biosensing technologies often face limitations in throughput and sensitivity.
• Localized surface plasmon resonance (LSPR) offers a promising label-free detection principle.

Purpose of the Study:

• To develop a novel and sensitive multi-throughput localized surface plasmon resonance (MLSPR) biosensor.
• To enable simultaneous detection of multiple acceptor-ligand interactions.
• To provide a convenient and label-free optical sensing platform.

Main Methods:

• Fabrication of an optical sensor using gold nanorods with varying aspect ratios.
• Construction of five distinct throughputs for MLSPR detection.
• Immobilization of the biosensor on glass slides for acceptor-ligand interaction analysis.
• Utilizing LSPR peaks in the 530-940nm wavelength range for simultaneous detection.

Main Results:

• Successful development of a novel MLSPR biosensor.
• Demonstration of simultaneous detection of multiple acceptor-ligand pairs.
• Achieved label-free detection of three antigen-antibody pairs.
• The sensor exhibits sensitivity and convenience due to the absence of labeling procedures.

Conclusions:

• The developed MLSPR biosensor is a sensitive and novel platform for simultaneous, label-free detection.
• The use of gold nanorods with different aspect ratios allows for multiplexed sensing.
• This biosensor offers a convenient and efficient method for analyzing biomolecular interactions, such as antigen-antibody pairs.