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Published on: November 7, 2018
[Extraction of praeruptorin A from Radix Peucedani by supercritical fluid extraction-CO2]
Ai-dong Liu1, Wei Hou, Xue-song Chen
1Department of Cardiology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun, Jilin Province 130021, China.
Objective:
To extract praeruptorin A from Radix Peucedani by supercritical fluid extraction (SFE)-CO2.
Methods:
After preliminary experiment, three main factors were acquired that could influence the result of SFE-CO2, including the time, pressure and temperature of the extraction. The optimal extraction process was carried out on orthogonal design, and SFE-CO2 was compared with the traditional methods.
Results:
In the extraction of the praeruptorin A, the best extraction conditions were 60 degrees C, 20 MPa, and duration for three hours. As cosolvent alcohol was added, the amount of extraction of the praeruptorin A increased, and the amount of SFE-CO2 extraction was higher than those of decoction and heating reflux.
Conclusion:
The SFE-CO2 in extracting praeruptorin A from Radix Peucedani is feasible and reliable.
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