[Glycine-dependent cryotransformation of Bacillus anthracis by DNA of various plasmids]

The possibility of the 1,8-60,0 Md plasmids DNA transfer into the cells of different Bacillus anthracis strains has been established. A number of parameters of the procedure has been optimized. The including of PEG6000 into the cryomixture in the final concentration 10% has been demonstrated to be vital for increase in transformants yields. Under the described conditions the maximal efficiency of transformation (1,2.10(3)) has been registered for the DNA of the plasmid pUB110. The efficiency of the process decreased concomittant with the size of the transformed plasmids representing 2-4.10(-1) for the plasmid perlicon pXO2 (60 Md). The plasmids obtained by Bacillus anthracis cells retained the functional and structural stability.