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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jun 27, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Using reverse genetics to develop small knockout collections for specific biological questions.

Julian Northey¹, Peter McCourt

¹Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|December 17, 2008

Summary

This summary is machine-generated.

Researchers can now create custom knockout gene collections in Arabidopsis using indexed insertion lines. This enables efficient hypothesis testing for various biological questions.

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Last Updated: Jun 27, 2026

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Published on: September 4, 2019

Area of Science:

Plant biology
Genetics
Molecular biology

Background:

Indexed mutagenized insertion lines provide a powerful resource for plant research.
Previous methods for gene knockout studies were less streamlined.

Purpose of the Study:

To outline a method for creating targeted gene knockout collections in Arabidopsis.
To facilitate hypothesis-driven research using these collections.

Main Methods:

Querying Arabidopsis databases to identify specific gene knockout lines.
Ordering and verifying the homozygosity of selected lines.
Assembling custom knockout collections.

Main Results:

A practical workflow for generating custom gene knockout collections is established.
These collections are readily usable for multiple rounds of scientific inquiry.

Conclusions:

The availability of indexed insertion lines simplifies the creation of custom knockout collections.
This approach enhances the efficiency and scope of hypothesis testing in Arabidopsis research.