Related Experiment Video
Updated: Jun 26, 2026
Multimodal Approach to Assess Bone Regeneration and Scaffold Performance
Published on: February 13, 2026
Quantification of bone tissue regeneration employing beta-tricalcium phosphate by three-dimensional non-invasive
Michael Stiller1, Alexander Rack, Simon Zabler
1Department of Maxillofacial and Facial-Plastic Surgery, Division of Oral Medicine, Radiology and Surgery, Charite-University Medical Center Berlin, Campus Benjamin Franklin, Assmannshauser Str. 4-6, 14197 Berlin, Germany. stiller@implant-consult.de
Purpose:
This methodical study presents a novel approach to evaluate the validity of two-dimensional histomorphometric measurements of a bone biopsy specimen after sinus floor elevation by means of high contrast, high resolution, three-dimensional and non-destructive synchrotron micro-tomography (SCT). The aim of this methodical description is to demonstrate the potential of this new approach for the evaluation of bone biopsy samples.
Materials And Methods:
Unilateral sinus grafting was carried out exemplarily in two patients using a combination of beta-tricalcium phosphate (beta-TCP) and autogenous bone chips. For the first patient a beta-TCP with 35% porosity and in the second with 60% porosity was used. At implant placement, 6 months after sinus grafting, a cylindrical specimen was biopsied from the augmented area. Subsequent to the histological embedding in resin the specimens were imaged using a SCT facility resulting in three-dimensional (3-D) images with approximately 4 microm spatial resolution (1.5 microm pixel size) for each patient's specimen. Subsequent to the SCT acquisition, tissue sections were prepared for histomorphometric analysis.
Results:
Bone area fractions determined by two-dimensional (2-D) quantitative histomorphometry and by analysis of the corresponding 2-D slice from the SCT volume data were similar. For the first biopsy specimen (beta-TCP with 35% porosity), the bone area fractions were 53.3% and 54.9% as derived by histomorphometry and by analyzing a SCT slice, respectively. For the second biopsy specimen (beta-TCP with 60% porosity) the bone area fractions were 38.8% and 39% respectively. Although the agreement between the 2-D methods was excellent, the area fractions were somewhat higher than the volume fractions computed by 3-D image analysis on the entire SCT volume data set. The volume fractions were 48.8% (first biopsy specimen) and 36.3% (second biopsy specimen).
Conclusion:
Although the agreement between the 2-D methods is excellent in terms of computing the area fractions, the structural 3-D insight which can be derived from classical 2-D methods, including histomorphometric analysis is considerably limited. This fact is emphasized by the discrepancy between the measured areas and volume fractions.
