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Published on: February 17, 2017
[Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse]
Ying Shi1, Fei-Li Wei, Ya-Li Liu
1Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
Objective:
To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.
Methods:
TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.
Results:
TAT-HBX-EGFP was highly expression in E. coli; HBX could be induced into mouse liver by TAT.
Conclusion:
HBX protein could be induced into mouse liver by TAT induced peptide.
