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Updated: Jun 26, 2026

Practical Considerations for the Design, Execution, and Interpretation of Studies Involving Whole-Bone Bending Tests of Rodent Bones
Published on: September 1, 2023
[Revealing mechanical property of intact rabbit tibia in three-point bending test]
Hong-Gang Zhong1, Wei-Hua Liu, Hai-Bin Bu
1College of Life Science & Bioengineering, Beijing University of Technology, Beijing 100022, China.
Objective:
To explore the mechanical property of intact long bone through three-point bending test of rabbit tibia according to mechanics of materials.
Methods:
Ten pairs of normal rabbits tibia were treated on three-point bending test with 80 mm supporting distance in order to corroborate the virtual loading test on bone model.
Results:
Bending stiffness and strength of 10 pairs of normal rabbit tibia were obtained. The maximum flexibility of the right and the left tibia was (2.737 +/- 0.262) mm and (2.739 +/- 0.233) mm respectively. The maximum load, which indicated the bending strength, of the right and the left tibia was (17.803 +/- 2.675) kg and (18.366 +/- 2.653) kg respectively. The area under the load-flexibility curve before fail of the right and the left tibia was (23.829 +/- 4.413) kg/mm and (24.725 +/- 4.101) kg x mm respectively. The slope of the load-flexibility curve which indicate the stiffness of the right and the left tibia was (7.545 +/- 1.310) kg/mm and (7.631 +/- 1.174) kg x mm respectively.
Conclusion:
The discrepancy between different animal body is larger than conventionally required. It's suggested for performing three-point bending test of long bone of the body both sides in pair.

