A rare GC mutant in horses disclosed by isoelectric focusing and subsequent immunoprinting

An apparent incompatibility in the GC/DBP system in a mare and her colt was found after classification by polyacrylamide gel electrophoresis. Because of this observation an analysis of the equine GC/DBP system by isoelectric focusing and subsequent immunoprinting was initiated. Further GC subtypes, however, were not encountered: the three phenotypes GC F, FS and S were also delineated by this method. The GC types in the case of disputed descent were dissolved: GC S was found in the mare and GC FS in her colt as well as in another of her male offspring. It is proposed that a rare GC mutant is responsible for this observation, namely a GC S* component which is readily classified by isoelectric focusing and immunoprinting and difficult to disclose by polyacrylamide gel electrophoresis.