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Updated: Jun 26, 2026
09:39
Mapping Dysfunctional Protein-Protein Interactions in Disease
Published on: October 24, 2025
Evolution of organelle-associated protein profiling
Wei Yan1, Ruedi Aebersold, Elaine W Raines
1Institute for Systems Biology, Seattle, WA 98103, USA.
Journal of Proteomics
|December 27, 2008
Summary
Proteomics technology has advanced organelle studies by identifying protein functions. Quantitative profiling methods overcome limitations, enabling new insights into protein movement and disease-related organelle changes.
Area of Science:
- Cell Biology
- Proteomics
- Biochemistry
Background:
- Understanding organelle protein composition is crucial for defining protein roles in cellular structure and function.
- Mass spectrometry-based proteomics has significantly advanced the identification of proteins within various organelles over the last decade.
Purpose of the Study:
- To review the evolution of organelle proteomics technology.
- To discuss how quantitative profiling approaches address limitations like organelle heterogeneity and purity.
- To highlight the potential of organelle profiling in understanding protein dynamics and disease.
Main Methods:
- Review of mass spectrometry-based proteomics techniques.
- Discussion of quantitative profiling strategies for organelle analysis.
- Analysis of technological advancements in organelle proteomics.
Main Results:
- Proteomics has enabled the association of numerous novel proteins with specific organelles.
- Quantitative profiling offers solutions to challenges in organelle purity and heterogeneity.
- The review chronicles the technological progression in the field of organelle proteomics.
Conclusions:
- Quantitative organelle profiling is a promising approach to study complex protein shuttling dynamics.
- This technology is critical for understanding disease-based alterations in organelle function.
- Further advancements are needed, but the potential impact on cell biology and disease research is substantial.
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