Nanoporous cerium oxide thin film for glucose biosensor

Area of Science:

• Materials Science
• Nanotechnology
• Biotechnology

Background:

• Development of efficient biosensors for glucose monitoring is crucial for diabetes management.
• Cerium oxide (CeO(2)) offers unique redox properties and biocompatibility for biosensor applications.
• Immobilization of enzymes like glucose oxidase (GOx) on suitable substrates is key for biosensor performance.

Purpose of the Study:

• To develop and characterize a novel nanoporous CeO(2) thin film for glucose oxidase immobilization.
• To evaluate the performance of the GOx/CeO(2)/Pt bio-electrode for glucose sensing.
• To investigate the enzyme kinetics and affinity of GOx immobilized on the nanoporous CeO(2) surface.

Main Methods:

• Pulsed laser deposition (PLD) was used to create nanoporous CeO(2) thin films on Pt-coated glass.
• Atomic force microscopy (AFM) was employed to analyze the surface morphology.
• Differential pulsed voltammetry (DPV) and optical measurements were used to assess glucose sensing capabilities.

Main Results:

• AFM confirmed a nanoporous surface morphology of the CeO(2) thin film.
• The GOx/CeO(2)/Pt bio-electrode exhibited linear glucose detection from 25-300 mg/dl.
• A low Michaelis-Menten constant (1.01 mM) indicated high enzyme affinity, suggesting effective GOx immobilization.

Conclusions:

• Nanoporous CeO(2) thin films provide an effective platform for glucose oxidase immobilization.
• The developed bio-electrode shows promising performance for glucose sensing without surface functionalization or mediators.
• This approach offers a potential advancement in non-enzymatic glucose detection technologies.