Related Experiment Video
Updated: Jun 26, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts
Published on: February 18, 2010
Vacuolar sorting behaviors of 11S globulins in plant cells
Takashi Mori1, Yohei Saruta, Takako Fukuda
1Laboratory of Food Quality Design and Development, Graduate School of Agriculture, Kyoto University.
Abstract:
Plant seed cells amass storage proteins that are synthesized on the endoplasmic reticulumn (ER) and then transported to protein storage vacuoles (PSVs). Many dicotyledonous seeds contain 11S globulin (11S) as a major storage protein. We investigated the accumulation behaviors of pea and pumpkin 11S during seed maturation and compared them with soybean 11S biogenesis (Mori et al., 2004). The accumulation of pea 11S in seeds was very similar to that of soybean 11S at all the development stages we examined, whereas pumpkin 11S condensed in the ER. The determinant of accumulation behavior might be the surface hydrophobicity of 11S. Further, we examined the accumulation of 11Ss in tobacco BY-2 cells to analyze behavior in the same environment. 11Ss expressed in BY2 cells were all observed in precursor form (pro11S). Pro11S with high surface hydrophobicity might be transported to vacuoles in a multivesicular body-mediated pathway when the expression level remains low.
Related Concept Videos
Overview of Protein Sorting and Transport
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation. In gated transport, folded...
Vesicular Tubular Clusters
With the help of motor proteins such...
Protein Transport to the Thylakoids
Protein Transport to the Inner Chloroplast Membrane
Short-distance Transport of Resources
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

