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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jun 26, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

MicroRNA detection by microarray.

Wei Li1, Kangcheng Ruan

  • 1State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China.

Analytical and Bioanalytical Chemistry
|January 10, 2009
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) regulate gene expression and are vital in biological processes and diseases. This review covers key miRNA detection technologies, focusing on microRNA arrays for profiling gene expression.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression.
  • They are crucial in cellular processes like differentiation, proliferation, and apoptosis.
  • Dysregulation of miRNAs is implicated in various disease states.

Purpose of the Study:

  • To review existing technologies for detecting microRNA expression.
  • To highlight the significance of microRNA profiling in life science research.
  • To emphasize microRNA arrays as a key detection method.

Main Methods:

  • Overview of established miRNA detection techniques.
  • Discussion of cloning and Northern blotting.
  • Detailed examination of real-time RT-PCR, microRNA arrays, and in situ hybridization (ISH).

Main Results:

  • Multiple methodologies exist for miRNA profiling.
  • MicroRNA arrays offer a significant approach for comprehensive expression analysis.
  • Each method has distinct advantages for specific research applications.

Conclusions:

  • Accurate detection of miRNA expression is fundamental for advancing miRNA research.
  • MicroRNA arrays provide a powerful tool for high-throughput miRNA profiling.
  • Continued development of detection technologies will accelerate discoveries in miRNA biology and disease.