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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A Novel Method for In Situ Electromechanical Characterization of Nanoscale Specimens
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In situ SEM indentation experiments: instruments, methodology, and applications.

Rudy Ghisleni1, Karolina Rzepiejewska-Malyska, Laetitia Philippe

  • 1Laboratory for Mechanics of Materials and Nanostructures, EMPA - Swiss Federal Laboratories for Materials Testing and Research, Feuerwerkerstrasse 39, CH-3602 Thun, Switzerland.

Microscopy Research and Technique
|January 14, 2009
PubMed
Summary

Two new in situ scanning electron microscope (SEM) indentation instruments were developed for microN to N load ranges. These instruments enable precise mechanical testing of thin films and nanowires, crucial for microelectromechanical systems (MEMS) and nanodevices.

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Area of Science:

  • Materials Science
  • Mechanical Engineering
  • Nanotechnology

Background:

  • In situ mechanical testing within a scanning electron microscope (SEM) offers high-resolution insights into material behavior.
  • Characterizing the mechanical properties of micro and nanoscale materials is essential for advanced device development.

Purpose of the Study:

  • To present the design and capabilities of two novel in situ SEM indentation instruments.
  • To demonstrate the utility of these instruments through applications on thin films and nanowires.
  • To highlight the suitability of electrodeposited cobalt for microelectromechanical systems (MEMS) and nanodevices.

Main Methods:

  • Development of two in situ SEM indentation systems with load ranges from microNewtons (µN) to Newtons (N).
  • Application of the instruments for indentation testing of electrodeposited cobalt thin films.
  • Application of the instruments for indentation testing of electrodeposited cobalt nanowires.

Main Results:

  • The developed instruments provide a versatile platform for in situ mechanical characterization across a wide load spectrum.
  • Successful indentation experiments on both thin films and nanowires demonstrate the instruments' capabilities.
  • The study validates the use of electrodeposited cobalt in MEMS and NEMS due to its mechanical properties.

Conclusions:

  • In situ SEM indentation is a powerful technique for evaluating micro and nanoscale material properties.
  • The presented instruments offer significant advantages for materials research and device development.
  • Electrodeposited cobalt shows promise for applications in MEMS and NEMS devices.