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Updated: Jun 26, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Collapsin response mediator protein-2 is a calmodulin-binding protein
Z Zhang1, V Majava, A Greffier
1Center of Innovative Research, Banyan Biomarkers Inc, 12805 Research Drive, Alachua, FL 32615, USA. zqzhang@banyanbio.com
Abstract:
Collapsin response mediator protein-2 (CRMP-2) plays a crucial role in axonal guidance and neurite outgrowth during neural development and regeneration. We have studied the interaction between calmodulin (CaM) and CRMP-2 and how Ca(2+)/CaM binding modulates the biological functions of CRMP-2. We have shown that CRMP-2 binds to CaM directly in a Ca(2+)-dependent manner. The CaM binding site of CRMP-2 is proposed to reside in the last helix of the folded domain, and in line with this, a synthesized peptide representing this helix bound to CaM. In addition, CaM binding inhibits a homotetrameric assembly of CRMP-2 and attenuates calpainmediated CRMP-2 proteolysis. Furthermore, a CaM antagonist reduces the number and length of process induced by CRMP-2 overexpression in HEK293 cells. Take together, our data suggest that CRMP-2 is a novel CaM-binding protein and that CaM binding may play an important role in regulating CRMP-2 functions.
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