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Related Concept Videos

Gas Chromatography: Types of Detectors-II01:19

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
IR Spectrometers01:25

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Gas Chromatography: Types of Detectors-I01:21

Gas Chromatography: Types of Detectors-I

There are different types of detectors used in gas chromatography, each with its own specific properties that make it suitable for detecting certain types of analytes. The most commonly used detectors in GC are thermal conductivity detector (TCD), flame ionization detector (FID), and electron capture detector (ECD).
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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Updated: Jun 26, 2026

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
09:48

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping

Published on: November 7, 2016

CCD based fiber-optic spectrometer detection.

Rakesh Kapoor1

  • 1Department of Physics, University of Alabama at Birmingham, Birmingham, AL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 20, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a compact, user-friendly fiber optic spectrometer for sensitive fluorescence detection in biosensors. This cost-effective tool enhances biotechnology research and applications.

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Last Updated: Jun 26, 2026

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping
09:48

Fiber Optic Distributed Sensors for High-resolution Temperature Field Mapping

Published on: November 7, 2016

Implementation of a Reference Interferometer for Nanodetection
16:11

Implementation of a Reference Interferometer for Nanodetection

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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

Area of Science:

  • Biotechnology
  • Spectroscopy
  • Sensor Technology

Background:

  • Biotechnology research demands sensitive and cost-effective measurement tools.
  • Fluorescence offers a simple, sensitive, and economical approach for biosensor techniques.
  • Spectrometers are crucial for spectroscopic measurements.

Purpose of the Study:

  • To describe the utilization of a charged coupled device (CCD)-based fiber optic spectrometer for fluorescence signal detection.
  • To highlight the application of this technology in fiber-optic-based sensors.
  • To demonstrate the adaptability of the method for broader fluorescence detection applications.

Main Methods:

  • Employing a charged coupled device (CCD)-based fiber optic spectrometer.
  • Detecting fluorescence signals from a fiber-optic-based sensor.
  • Utilizing spectroscopy for sensitive measurements.

Main Results:

  • Demonstrated the successful use of a CCD-based fiber optic spectrometer for fluorescence detection.
  • Showcased a compact, lightweight, and easy-to-use spectroscopic tool.
  • Confirmed the cost-effectiveness and high sensitivity of the fluorescence measurement method.

Conclusions:

  • CCD-based fiber optic spectrometers provide a sensitive and cost-effective solution for fluorescence detection.
  • The described method is highly applicable to fiber-optic-based biosensors.
  • This technique can be readily extended to various other fluorescence detection applications in biotechnology.