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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 26, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Identifying small pulsatile signals within noisy data: a fluorescence application.

Michael L Johnson¹, Leon S Farhy, Paula P Veldhuis

¹Department of Pharmacology, University of Virginia Health System, Charlottesville, Virginia, USA.

Methods in Enzymology

|January 21, 2009

Summary

This summary is machine-generated.

This study shows AutoDecon can reliably detect single events, like a single fluorophore, even with noisy data. This method is useful for analyzing pulsatile signals in various scientific fields.

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2026-06-19T13:36:16.374505+00:00

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