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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

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Published on: March 17, 2023

[A study on surface plasmon resonance-based gene chip].

Dayong Gu¹, Lei Shi, Huawei Yu

¹Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi

|January 27, 2009

Summary

This summary is machine-generated.

This study optimized a surface plasmon resonance (SPR)-based gene chip using nanogold films and self-assembled monolayers. The developed gene chip demonstrates high sensitivity and specificity for accurate base mismatch detection in SPR systems.

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Published on: July 21, 2014

Area of Science:

Nanotechnology
Biotechnology
Analytical Chemistry

Background:

Surface Plasmon Resonance (SPR) is a label-free optical detection method.
Gene chips are crucial for DNA analysis and diagnostics.
Developing sensitive and specific gene detection platforms is essential.

Purpose of the Study:

To construct and optimize a novel SPR-based gene chip.
To enhance the sensitivity and specificity of the gene chip for base mismatch detection.
To evaluate the performance of the optimized gene chip in an SPR detection system.

Main Methods:

Synthesis of nanogold film using Frens' method and plating on chip via Chlorauric acid/hydroxylamine method.
Immobilization of probes onto the nanogold film using Self-Assembled Monolayer (SAM) technology.
Optimization of probe fixing time, concentration, chip sensitivity, and specificity.

Main Results:

A 2.5 nm nanogold film thickness yielded optimal SPR reflection.
Optimal probe immobilization achieved with 4.5 hours fixation at 1500 nmol/L concentration.
The optimized gene chip demonstrated high sensitivity and specificity, accurately detecting base mismatches.

Conclusions:

A highly efficient SPR-based gene chip was successfully constructed and optimized.
The developed gene chip offers a sensitive and specific platform for SPR sensor detection.
This technology has potential applications in genetic analysis and diagnostics.