pH programming in capillary electrophoresis by means of temperature programming
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Updated: Jun 26, 2026

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
1Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, MB Eindhoven, The Netherlands. j.c.reijenga@tue.nl
Abstract:
When using a background electrolyte with a buffer having strong temperature dependence of pK, different operating temperatures result in different operating pH values, using the same background electrolyte (BGE). It has been shown that this can be used to fine-tune selectivities of sample mixtures of weak analytes. Capillary electrophoresis (CE) equipment is designed to operate under isothermal conditions, but by proper programming, a very reproducible temperature and thus BGE pH program during the analysis can be realized. This was experimentally verified and illustrated by computer simulations. Equipment characteristics have been determined, and possibilities and restrictions to make use of this feature are presented.
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