[Inductive effect of RNAi RelB dendritic cells on the CD4(+)CD25(+) T cells proliferation]

To discover the inductive proliferation effect of the RNAi RelB dendritic cells (DCs) on the heterogenic CD4(+)CD25(+) T cells in mice.

The proportion of CD4(+)CD25(+) T cells were quantification through flow cytometer when each group of DCs interact with heterogenic T cells in mixed lymphocyte reaction, including control DCs, LPS stimulated DCs, RNAi RelB DCs and LPS stimulated RNAi RelB DCs.

Control DCs stimulate more CD4(+)CD25(+) T cells to proliferate than LPS stimulated DCs(P<0.01). RNAi RelB DCs and LPS stimulated RNAi RelB DCs significantly improved CD4(+)CD25(+) T cells proliferation compared with LPS stimulated DCs(P<0.01). RNAi RelB DCs can also induced CD4(+)CD25(+) T cells to proliferate more than Control DCs (P<0.05).

RNAi RelB DCs possess powerful potential to induce CD4(+)CD25(+) T cells proliferation, one of characteristics of immature dendritic cells. This type of RNAi RelB DCs can be used as tolerogenic DCs to regulate autoimmunity and other immune responses.