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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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Directed evolution of homing endonuclease I-SceI with altered sequence specificity

Zhilei Chen1, Fei Wen, Ning Sun

  • 1Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Protein Engineering, Design & Selection : PEDS
|January 30, 2009
PubMed
Summary

Researchers engineered homing endonucleases for genome editing. A novel selection system evolved mutant I-SceI enzymes with enhanced DNA cleavage activity for new targets, improving genome targeting efficiency.

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