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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Jun 25, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Two-dimensional protein separation in microfluidic devices.

Hong Chen1, Z Hugh Fan

  • 1Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.

Electrophoresis
|February 7, 2009
PubMed
Summary
This summary is machine-generated.

Microfluidic devices offer a promising alternative for two-dimensional electrophoresis (2-DE) in proteomics, addressing the time and reproducibility issues of conventional methods. This review explores microfluidic 2-DE approaches for faster, high-resolution separations in drug discovery and diagnostics.

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

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Last Updated: Jun 25, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Proteomics is crucial for drug discovery and medical diagnostics.
  • Conventional two-dimensional electrophoresis (2-DE) is time-consuming and lacks reproducibility.
  • There is a need for faster, high-resolution separation techniques in proteomics.

Purpose of the Study:

  • To review advancements in microfluidic two-dimensional electrophoresis (2-DE).
  • To explore different microfluidic 2-DE configurations.
  • To discuss integration of microvalves for improved separation.

Main Methods:

  • Review of literature on microfluidic 2-DE devices.
  • Analysis of configurations: single channel, intersected channels, and multi-channel systems.
  • Discussion on microvalve integration for contamination prevention.

Main Results:

  • Microfluidic 2-DE enables separation in various channel configurations.
  • Microvalve integration is essential for preventing cross-contamination.
  • Microfluidic 2-DE shows potential for improved speed and resolution.

Conclusions:

  • Microfluidic 2-DE addresses limitations of conventional methods.
  • Further development is needed to match conventional 2-DE performance.
  • Microfluidic 2-DE holds potential as a future alternative in proteomics.