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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

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Updated: Jun 25, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Correlative light and electron microscopy.

Minoo Razi1, Sharon A Tooze

  • 1London Research Institute, Cancer Research UK, London, UK.

Methods in Enzymology
|February 10, 2009
PubMed
Summary
This summary is machine-generated.

Correlative light and electron microscopy (CLEM) monitors cellular autophagy by combining broad overviews with high-resolution imaging. This morphological technique enhances the study of autophagosome formation and function for cellular homeostasis.

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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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Last Updated: Jun 25, 2026

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Published on: August 7, 2016

Epon Post Embedding Correlative Light and Electron Microscopy
08:47

Epon Post Embedding Correlative Light and Electron Microscopy

Published on: January 12, 2024

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Area of Science:

  • Cell Biology
  • Molecular Biology

Background:

  • Autophagy is a vital cellular process for maintaining homeostasis, involving the formation of autophagosomes for degradation within lysosomes.
  • Efficient autophagy requires functional autophagosomes and lysosomal machinery to recycle cellular components.
  • Existing methods to monitor autophagy include biochemical, morphological, quantitative, and qualitative assays.

Purpose of the Study:

  • To describe a novel method for monitoring the autophagic process using correlative light and electron microscopy (CLEM).
  • To demonstrate the utility of CLEM for studying autophagosome formation and function in various cell and tissue types.

Main Methods:

  • The study employed correlative light and electron microscopy (CLEM) for morphological analysis of autophagy.
  • CLEM integrates light microscopy (LM) and electron microscopy (EM) on the same sample.
  • Samples were prepared on gridded coverslips compatible with both LM and EM.

Main Results:

  • CLEM provides a broad, low-magnification overview of cellular events, enabling spatial and temporal assessments.
  • High-resolution EM imaging within CLEM allows detailed examination of individual autophagosomes and cellular compartments.
  • The method is applicable to diverse cell and tissue samples.

Conclusions:

  • CLEM offers an advanced approach to studying autophagy, surpassing the limitations of LM or EM alone.
  • This technique facilitates a comprehensive understanding of autophagosome dynamics and their role in cellular homeostasis.
  • CLEM is a versatile tool for researchers investigating the intricate mechanisms of autophagy.