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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
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Published on: February 10, 2014

Biofunctional subwavelength optical waveguides for biodetection.

Donald J Sirbuly1, Nicholas O Fischer, Shih-Chieh J Huang

  • 1Chemistry, Materials, Earth, and Life Sciences Directorate, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, California 94550, USA. sirbuly2@llnl.gov

ACS Nano
|February 12, 2009
PubMed
Summary
This summary is machine-generated.

We developed a novel biofunctional photonic device for real-time biological molecule detection. This platform uses lipid bilayers on nanowire waveguides for sensitive and rapid DNA hybridization sensing.

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Area of Science:

  • Photonics
  • Biotechnology
  • Nanotechnology

Background:

  • Developing sensitive and real-time biosensors is crucial for molecular diagnostics.
  • Existing biosensing platforms often face challenges with stability and reusability.

Purpose of the Study:

  • To create a versatile biofunctional subwavelength photonic device platform for detecting biological molecules.
  • To demonstrate the utility of lipid bilayers integrated with optical waveguides for biosensing.

Main Methods:

  • Fabrication of devices with lipid bilayer membranes fused onto metal oxide nanowire waveguides.
  • Utilizing an evanescent field within an optical cavity to interact with receptors on the lipid bilayer.
  • Assessing lipid bilayer properties (continuity, mobile fraction, fouling resistance) and membrane exchange capability.
  • Detecting DNA target sequence hybridization to probe DNA strands via optical sensing.

Main Results:

  • The fabricated lipid bilayers were continuous, highly mobile, and resistant to fouling.
  • The platform demonstrated rapid membrane exchange.
  • Successful real-time detection of specific DNA hybridization events was achieved.

Conclusions:

  • The biofunctional photonic device platform enables versatile and real-time detection of biological molecules.
  • The evanescent wave sensing architecture is promising for developing portable, all-optical detection systems.