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Updated: Jun 25, 2026

Interview: Protein Folding and Studies of Neurodegenerative Diseases
Published on: July 16, 2008
What have we learned from the studies of two-state folders, and what are the unanswered questions about two-state
1T C Department of Biophysics, The Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA. barrick@jhu.edu
Abstract:
Small proteins with globular structures often fold by simple all-or-none mechanisms, both in an equilibrium and a kinetic sense, despite the very large number of partly folded conformations available. This type of 'two-state' folding will be discussed in terms of experimental tests, underlying molecular mechanisms, and limits to two-state behavior. Factors that appear to be important for two-state folding include topology (sequence distance of contacts in the native structure), molecular cooperativity and local energy distribution. Because their local stability distributions and cooperativities can be dissected and analyzed separately from topological features, recent studies of the folding of symmetric proteins will be discussed as a means to better understand the origins of two-state folding.
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