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Nipah virus edits its P gene at high frequency to express the V and W proteins

Sachin Kulkarni1, Valentina Volchkova, Christopher F Basler

  • 1Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA.

Journal of Virology
|February 13, 2009
PubMed

Insights

Nipah virus (NiV) uses extensive mRNA editing to express its V and W proteins. This process is crucial for viral protein production during NiV infection.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Nipah virus (NiV) is a highly pathogenic paramyxovirus.
  • The NiV P gene is known to produce multiple proteins, including P, V, W, and C, through mechanisms like mRNA editing and alternate reading frames.

Purpose of the Study:

  • To confirm the expression of V, W, and C proteins in NiV-infected cells.
  • To analyze the mRNA editing patterns and transcript ratios of the NiV P gene.

Main Methods:

  • Utilized specific antibodies to detect viral proteins in infected cells.
  • Analyzed P-gene transcripts to determine mRNA editing frequency and transcript ratios.

Main Results:

  • Confirmed the expression of V, W, and C proteins in NiV-infected cells.
  • Observed a high frequency of mRNA editing (82%) in NiV transcripts, with up to 11 G insertions.
  • Found that the ratio of P:V:W mRNA transcripts changes over time, with increased V and W proportions during infection progression.

Conclusions:

  • The high frequency of mRNA editing in NiV transcripts is essential for the expression of V and W proteins.
  • These findings provide insights into the complex gene expression strategy of Nipah virus.

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