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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 25, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Integrating accelerated tryptic digestion into proteomics workflows.

Gordon W Slysz1, David C Schriemer

  • 1Department of Chemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid microscale trypsin digestion method for proteins, achieving high sequence coverage in seconds. The approach integrates seamlessly with liquid chromatography for efficient protein analysis.

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Last Updated: Jun 25, 2026

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Published on: September 15, 2015

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Protein identification relies on efficient enzymatic digestion.
  • Traditional digestion methods can be time-consuming and require significant enzyme quantities.
  • Integrating digestion with separation techniques is desirable for streamlined workflows.

Purpose of the Study:

  • To develop an accelerated protein digestion procedure using a microscale trypsin cartridge.
  • To enable online integration of this digestion method with reversed-phase protein separation.
  • To describe the construction and operation of effective digestor cartridges and fluidic systems.

Main Methods:

  • Utilized a microscale trypsin cartridge under aqueous-organic conditions for accelerated digestion.
  • Developed a fluidic system for online integration of digestion after chromatographic separation.
  • Implemented dynamic effluent titration by coordinating gradient systems to maintain stable pH and solvent levels.

Main Results:

  • Achieved high sequence coverage protein digestions in seconds with minimal enzyme.
  • Demonstrated successful online integration of digestion with reversed-phase protein separation.
  • Enabled digestion and identification of subnanogram levels of protein.

Conclusions:

  • The microscale trypsin cartridge offers a rapid and efficient method for protein digestion.
  • The described fluidic system facilitates seamless integration of digestion into chromatographic workflows.
  • This approach significantly enhances throughput for proteomic analyses.