Cadmium induction of metallothioneins in several dogfish organs

Dogfish Scyliorhinus canicula were exposed to 50 mg/l Cd for 4 days for inducing metallothionein synthesis. Spleen, pancreas, kidney and gonads were dissected out and metallothionein presence was checked by means of gel filtration chromatography in Sephadex G-75 and sodium dodecyl sulphate polyacrylamide electrophoresis. In pancreas and kidney, a cadmium-binding protein with spectroscopical and electrophoretic properties similar to those of dogfish liver metallothionein was found. In the other organs, the existence of an analogue protein but at very low concentrations is feasible.