[AQP1 expression in vascular endothelial cells in vitro under hypoxia conditions]

To investigate the expression of AQP1 in endothelial cells under hypoxia conditions in vitro.

The cultured human umbilical vein endothelial cells were randomly assigned to hypoxia groups (exposed to 1% of O2 for 3 h, 6 h, 12 h and 24 h respectively), and control group (ordinary culture). The expressions of AQP1 mRNA and protein were detected by real time PCR and immunohistochemistry respectively.

The expressions of AQP1 protein and mRNA increased after exposure to hypoxia initially and then decreased afterwards.

Hypoxia regulates the expression of AQP1 in vascular endothelial cells.