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Updated: Jun 25, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
High Resolution Separations and Improved Ion Production and Transmission in Metabolomics
Thomas O Metz1, Jason S Page, Erin S Baker
1Biological Sciences Division Pacific Northwest National Laboratory Richland, WA.
Abstract:
The goal of metabolomics analyses is the detection and quantitation of as many sample components as reasonably possible in order to identify compounds or "features" that can be used to characterize the samples under study. When utilizing electrospray ionization to produce ions for analysis by mass spectrometry (MS), it is important that metabolome sample constituents be efficiently separated prior to ion production, in order to minimize ionization suppression and thereby extend the dynamic range of the measurement, as well as the coverage of the metabolome. Similarly, optimization of the MS inlet and interface can lead to increased measurement sensitivity. This perspective review will focus on the role of high resolution liquid chromatography (LC) separations in conjunction with improved ion production and transmission for LC-MS-based metabolomics. Additional emphasis will be placed on the compromise between metabolome coverage and sample analysis throughput.
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