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Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Purification of inactive precursor of carboxypeptidase Y using selective cleavage method coupled with molecular
Haruko Maeda1, Mitsuru Nagayama, Kouichi Kuroda
1Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan.
Bioscience, Biotechnology, and Biochemistry
|March 10, 2009
Abstract:
A novel purification system for inactive precursor proteins without conventional time-consuming purification steps was established. We purified an inactive precursor protein, procarboxypeptidase Y (proCPY), which was displayed on the cell surface of yeast, due to difficulty of purifying it by a system in which it is produced in cells.
